Optical Trapping

An optical trap or “optical tweezers” is a device which can apply and measure piconewton sized forces on micron sized dielectric objects under a microscope using a highly focused light beam. It allows very detailed manipulations and measurements of several interesting systems in the fields of molecular and cell biology and thus acts as a major tool in biophysics. They are used in biological experiments ranging from cell sorting to the unzipping of DNA. Similar principles are also used in physical applications such as atom cooling. In this experiment, you will measure the Brownian motion of a trapped silica microsphere in aqueous solution, both testing the theory of statistical mechanics and calibrating the “spring constant” of the trap. Then, using the calibrated trap, you will measure forces in biological systems, such as the actin-myosin molecular motors of vesicle transport in onion cells, the E. coli flagellar motor, or the restoring force of a stretched DNA molecule. In its present form, large portions of this lab guide are derived from the literature for MIT Bioenhineering subject 20.309 [1] and UC Berkeley Physics subject Physics 111 Lab [2].